› 6cja

X-ray diffraction
1.7Å resolution

Crystal structure of Cystathionine beta-lyase from Legionella pneumophila Philadelphia 1 in complex with Alanyl-PLP and Serine

Released:
DOI: 10.2210/pdb6cja/pdb
PDB entry 6cja coloured by chain, front view.
PDB entry 6cja coloured by chain, side view.
PDB entry 6cja coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 43.06 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q5ZX43 (Residues: 1-383; Coverage: 100%)
Gene names: lpg0890, metC
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments

3 bound ligands:
Ligand F0G 4 x F0G
Ligand SER 4 x SER
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 96.17Å b: 97.11Å c: 175.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.141 0.168
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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