› 7b1l

X-ray diffraction
1.85Å resolution

Crystal structure of phosphatidyl serine synthase (PSS) in the closed conformation with bound citrate.

Released:
DOI: 10.2210/pdb7b1l/pdb
PDB entry 7b1l coloured by chain, front view.
PDB entry 7b1l coloured by chain, side view.
PDB entry 7b1l coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Crystal structures of phosphatidyl serine synthase PSS reveal the catalytic mechanism of CDP-DAG alcohol O-phosphatidyl transferases
Centola M, Betz H, Yildiz O
Nat Commun 12 6982- (2021)

Function and Biology Details

Reaction catalysed: 
CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-diacylglycerol--serine O-phosphatidyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.95 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58609 (Residues: 1-201; Coverage: 100%)
Gene names: MJ1212, pssA
Sequence domains: CDP-alcohol phosphatidyltransferase

Ligands and Environments

7 bound ligands:
Ligand 58A 2 x 58A
Ligand CA 2 x CA
Ligand CL 6 x CL
Ligand SER 4 x SER
Ligand NA 2 x NA
Ligand MG 2 x MG
Ligand OLC 8 x OLC
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Unit cell:
a: 61.63Å b: 79.48Å c: 95.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.263 0.301
Expression system: Escherichia coli

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