Abstract
We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by agarose gel electrophoresis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA Primers
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DNA-Binding Proteins / genetics
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Genetic Linkage
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Genetics, Medical*
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Humans
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Kruppel-Like Transcription Factors
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Primates / genetics*
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Sex Determination Processes*
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Transcription Factors
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X Chromosome
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Y Chromosome
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Zinc Fingers
Substances
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DNA Primers
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DNA-Binding Proteins
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Kruppel-Like Transcription Factors
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Transcription Factors
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ZFY protein, human
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zinc finger protein, X-linked