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Antifungal Drug Resistance

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Damian J. Krysan

    1. Department of Pediatrics, University of Iowa, Iowa City, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. W. Scott Moye-Rowley

    1. Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2658)

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About this book

This detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic and molecular mechanisms of these alterations of antifungal drug susceptibility, and, finally, to approach the study of these processes in animal models of fungal infection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antifungal Drug Resistance: Methods and Protocols serves as an ideal resource for the field and a guide for investigators to study this important translational aspect of fungal biology.

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
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  2. Identification and Characterization of Molecules with Antifungal Activity

    1. Front Matter

      Pages 1-1
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    2. Antifungal Susceptibility of Yeasts and Filamentous Fungi by CLSI Broth Microdilution Testing

      • Nathan P. Wiederhold
      Pages 3-16

    3. Luciferase-Based High-Throughput Screen with Aspergillus fumigatus to Identify Antifungal Small Molecules

      • Timothy J. Opperman, Sourabh Dhingra, Cecilia Gutierrez-Perez, Steven M. Kwasny, Robert A. Cramer
      Pages 17-34

    4. A Dual-Readout High-Throughput Screening Assay for Small Molecules Active Against Aspergillus Fumigatus

      • Sarah R. Beattie, Damian J. Krysan
      Pages 35-42

    5. Antifungal Susceptibility Testing and Drug Discovery in the Dimorphic Fungus Histoplasma Capsulatum

      • Chad A. Rappleye
      Pages 43-52

    6. A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High-Throughput Screening

      • Olabayo H. Ajetunmobi, Gina Wall, Bruna V. Bonifacio, Daniel Montelongo-Jauregui, Jose L. Lopez-Ribot
      Pages 53-64

  3. Identification and Characterization of Fungal Strains with Altered Drug Susceptibility

    1. Front Matter

      Pages 65-65
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    2. Protocols for Measuring Tolerant and Heteroresistant Drug Responses of Pathogenic Yeasts

      • Naomi Lyons, Judith Berman
      Pages 67-79

    3. Genome-Wide Identification of Variants Associated with Antifungal Drug Resistance

      • Aina Martinez-Zurita, Christina A. Cuomo
      Pages 81-103

    4. Copy Number Variation and Allele Ratio Analysis in Candida albicans Using Whole Genome Sequencing Data

      • Robert T. Todd, Anna Selmecki
      Pages 105-125

    5. Drug Target Elucidation Through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans

      • Michael J. Hoy, Joseph Heitman
      Pages 127-143

    6. Chemical-Genetic Approaches for Exploring Mode of Action of Antifungal Compounds in the Fungal Pathogen Candida albicans

      • Nicole Robbins, Troy Ketela, Sang Hu Kim, Leah E. Cowen
      Pages 145-165

  4. Characterization of the Genetic and Molecular Mechanisms of Antifungal Resistance

    1. Front Matter

      Pages 167-167
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    2. Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance

      • Lucia Simonicova, W. Scott Moye-Rowley
      Pages 169-179

    3. Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae

      • Kelley R. Healey, Ashutosh Singh
      Pages 181-190

    4. Conditional Protein Depletion in the Analysis of Antifungal Drug Resistance in Candida glabrata

      • Thomas P. Conway, W. Scott Moye-Rowley
      Pages 191-200

    5. Transport across Membranes: Techniques for Measuring Efflux in Fungal Cells

      • Brooke D. Esquivel, Theodore C. White
      Pages 201-213

    6. Transport Across Membranes: Techniques for Measuring Drug Import in Fungal Cells

      • Brooke D. Esquivel, Theodore C. White
      Pages 215-221

  5. Characterization of Antifungal Drug Resistance Using Animal Infection Models

    1. Front Matter

      Pages 223-223
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Editors and Affiliations

  • Department of Pediatrics, University of Iowa, Iowa City, USA

    Damian J. Krysan

  • Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, USA

    W. Scott Moye-Rowley

Bibliographic Information

  • Book Title: Antifungal Drug Resistance

  • Book Subtitle: Methods and Protocols

  • Editors: Damian J. Krysan, W. Scott Moye-Rowley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3155-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3154-6Published: 07 April 2023

  • Softcover ISBN: 978-1-0716-3157-7Published: 07 April 2023

  • eBook ISBN: 978-1-0716-3155-3Published: 06 April 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 250

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Pharmacology/Toxicology, Medical Microbiology, Mycology

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