Cardiovascular disease remains the leading cause of morbidity and mortality in the
developed world. In recent decades, extraordinary effort has been devoted to defining the …

The rapid development of genome editing technology has brought major breakthroughs in
the fields of life science and medicine. In recent years, the clustered regularly interspaced …

Genome‐editing technology has emerged as a powerful method that enables the generation
of genetically modified cells and organisms necessary to elucidate gene function and …

Many novel CRISPR-based genome-editing tools, with a wide variety of applications, have
been developed in the past few years. The original CRISPR–Cas9 system was developed …

Cardiovascular diseases are among the main causes of morbidity and mortality in Western
countries and considered as a leading public health issue. Therefore, there is a strong need …

A variety of genetic cardiovascular diseases may one day be curable using gene editing
technology. Germline genome editing and correction promises to permanently remove …

Importance Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-
associated protein 9 (CRISPR-Cas9) has garnered a great degree of attention since its first …

Genome-editing tools, which include zinc finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs), and clustered regularly interspaced short palindromic …

Genetic engineering of model organisms and cultured cells has for decades provided
important insights into the mechanisms underlying cardiovascular development and …

The zebrafish (Danio rerio) has become a popular vertebrate model organism to study organ
formation and function due to its optical clarity and rapid embryonic development. The use of …