UniProtKB - P07997 (HEM1_CHICK)
UniProt
P07997 - HEM1_CHICK
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Protein
5-aminolevulinate synthase, nonspecific, mitochondrial
Gene
ALAS1
Organism
Gallus gallus (Chicken)
Status
Functioni
Catalytic activityi
Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactori
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.Proteins known to be involved in this subpathway in this organism are:
- 5-aminolevulinate synthase, erythroid-specific, mitochondrial (ALAS2), 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1), 5-aminolevulinate synthase (ALAS1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | Actions |
---|---|---|---|---|---|---|
Binding sitei | 212 – 212 | 1 | SubstrateBy similarity | |||
Binding sitei | 329 – 329 | 1 | SubstrateBy similarity | |||
Binding sitei | 348 – 348 | 1 | SubstrateBy similarity | |||
Binding sitei | 381 – 381 | 1 | Pyridoxal phosphateBy similarity | |||
Binding sitei | 409 – 409 | 1 | Pyridoxal phosphateBy similarity | |||
Binding sitei | 437 – 437 | 1 | Pyridoxal phosphateBy similarity | |||
Active sitei | 440 – 440 | 1 | By similarity | |||
Binding sitei | 469 – 469 | 1 | Pyridoxal phosphate; shared with dimeric partnerBy similarity | |||
Binding sitei | 470 – 470 | 1 | Pyridoxal phosphate; shared with dimeric partnerBy similarity | |||
Binding sitei | 557 – 557 | 1 | SubstrateBy similarity |
GO - Molecular functioni
- 5-aminolevulinate synthase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- heme biosynthetic process Source: Reactome
- heme metabolic process Source: Reactome
- protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
- small molecule metabolic process Source: Reactome
Keywords - Molecular functioni
Acyltransferase, TransferaseKeywords - Biological processi
Heme biosynthesisKeywords - Ligandi
Pyridoxal phosphateEnzyme and pathway databases
Reactomei | R-GGA-421525. Heme synthesis. |
UniPathwayi | UPA00251; UER00375. |
Names & Taxonomyi
Protein namesi | Recommended name: 5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)Short name: ALAS-H Alternative name(s): 5-aminolevulinic acid synthase 1 Delta-ALA synthase 1 Delta-aminolevulinate synthase 1 |
Gene namesi | Name:ALAS1 Synonyms:ALASN |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi | UP000000539: Unplaced Componenti |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: Reactome
- mitochondrial matrix Source: Reactome
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | Actions |
---|---|---|---|---|---|---|
Transit peptidei | 1 – 56 | 56 | Mitochondrion | Add BLAST | ||
Chaini | 57 – 635 | 579 | 5-aminolevulinate synthase, nonspecific, mitochondrial | PRO_0000001233 | Add BLAST |
Amino acid modifications
Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | Actions |
---|---|---|---|---|---|---|
Modified residuei | 440 – 440 | 1 | N6-(pyridoxal phosphate)lysineBy similarity |
Interactioni
Subunit structurei
Homodimer.
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
HOVERGENi | HBG005954. |
InParanoidi | P07997. |
KOi | K00643. |
PhylomeDBi | P07997. |
Family and domain databases
Gene3Di | 3.40.640.10. 1 hit. 3.90.1150.10. 1 hit. |
InterProi | IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR015118. 5aminolev_synth_preseq. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view] |
Pfami | PF00155. Aminotran_1_2. 1 hit. PF09029. Preseq_ALAS. 1 hit. [Graphical view] |
SUPFAMi | SSF53383. SSF53383. 1 hit. |
TIGRFAMsi | TIGR01821. 5aminolev_synth. 1 hit. |
PROSITEi | PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view] |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07997-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAVVRRCPF LARVSQAFLQ KAGPSLLFYA QHCPKMMEAA PPAAARGLAT
60 70 80 90 100
SASRGQQVEE TPAAQPEAKK AKEVAQQNTD GSQPPAGHPP AAAVQSSATK
110 120 130 140 150
CPFLAAQMNH KSSNVFCKAS LELQEDVKEM QVDRKGKEFA KIPTNSVVRN
160 170 180 190 200
TEAEGEEQSG LLKKFKDIML KQRPESVSHL LQDNLPKSVS TFQYDQFFEK
210 220 230 240 250
KIDEKKKDHT YRVFKTVNRK AQIFPMADDY SDSLITKKEV SVWCSNDYLG
260 270 280 290 300
MSRHPRVCGA VMDTLKQHGA GAGGTRNISG TSKFHVDLEK ELADLHGKDA
310 320 330 340 350
ALLFSSCFVA NDSTLFTLAK MLPGCEIYSD SGNHASMIQG IRNSRVPKHI
360 370 380 390 400
FRHNDVNHLR ELLKKSDPST PKIVAFETVH SMDGAVCPLE ELCDVAHEHG
410 420 430 440 450
AITFVDEVHA VGLYGARGGG IGDRDGVMHK MDIISGTLGK AFACVGGYIS
460 470 480 490 500
STSALIDTVR SYAAGFIFTT SLPPMLLAGA LESVRTLKSA EGQVLRRQHQ
510 520 530 540 550
RNVKLMRQML MDAGLPVVHC PSHIIPIRVA DAAKNTEICD KLMSQHSIYV
560 570 580 590 600
QAINYPTVPR GEELLRIAPT PHHTPQMMSY FLEKLLATWK DVGLELKPHS
610 620 630
SAECNFCRRP LHFEVMSERE RSYFSGMSKL LSVSA
Experimental Info
Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | Actions |
---|---|---|---|---|---|---|
Sequence conflicti | 53 – 53 | 1 | S → A in CAA26595 (PubMed:3839458).Curated | |||
Sequence conflicti | 53 – 53 | 1 | S → A no nucleotide entry (PubMed:2915978).Curated |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02827 mRNA. Translation: CAA26595.1. X03517, X03627 Genomic DNA. Translation: CAA27223.1. |
PIRi | A23538. SYCHAL. |
RefSeqi | NP_001018012.1. NM_001018012.1. |
UniGenei | Gga.1399. |
Genome annotation databases
GeneIDi | 552895. |
KEGGi | gga:552895. |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02827 mRNA. Translation: CAA26595.1. X03517, X03627 Genomic DNA. Translation: CAA27223.1. |
PIRi | A23538. SYCHAL. |
RefSeqi | NP_001018012.1. NM_001018012.1. |
UniGenei | Gga.1399. |
3D structure databases
ProteinModelPortali | P07997. |
ModBasei | Search... |
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
GeneIDi | 552895. |
KEGGi | gga:552895. |
Organism-specific databases
CTDi | 211. |
Phylogenomic databases
HOVERGENi | HBG005954. |
InParanoidi | P07997. |
KOi | K00643. |
PhylomeDBi | P07997. |
Enzyme and pathway databases
UniPathwayi | UPA00251; UER00375. |
Reactomei | R-GGA-421525. Heme synthesis. |
Miscellaneous databases
NextBioi | 20879866. |
PROi | P07997. |
Family and domain databases
Gene3Di | 3.40.640.10. 1 hit. 3.90.1150.10. 1 hit. |
InterProi | IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR015118. 5aminolev_synth_preseq. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view] |
Pfami | PF00155. Aminotran_1_2. 1 hit. PF09029. Preseq_ALAS. 1 hit. [Graphical view] |
SUPFAMi | SSF53383. SSF53383. 1 hit. |
TIGRFAMsi | TIGR01821. 5aminolev_synth. 1 hit. |
PROSITEi | PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view] |
ProtoNeti | Search... |
Publicationsi
- "Complete nucleotide sequence of hepatic 5-aminolaevulinate synthase precursor."
Borthwick I.A., Srivastava G., Day A.R., Pirola B.A., Snoswell M.A., May B.K., Elliott W.H.
Eur. J. Biochem. 150:481-484(1985) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA].Tissue: Liver. - "Nucleotide sequence of the chicken 5-aminolevulinate synthase gene."
Maguire D.J., Day A.R., Borthwick I.A., Srivastava G., Wigley P.L., May B.K., Elliott W.H.
Nucleic Acids Res. 14:1379-1391(1986) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].Tissue: Liver. - "Expression of delta-aminolevulinate synthase in avian cells: separate genes encode erythroid-specific and nonspecific isozymes."
Riddle R.D., Yamamoto M., Engel J.D.
Proc. Natl. Acad. Sci. U.S.A. 86:792-796(1989) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE.
Entry informationi
Entry namei | HEM1_CHICK | ||||||||
Accessioni | P07997Primary (citable) accession number: P07997 | ||||||||
Entry historyi |
| ||||||||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Miscellaneous
There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- PATHWAY commentsIndex of metabolic and biosynthesis pathways
- SIMILARITY commentsIndex of protein domains and families
Similar proteinsi
Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:100% | UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry. |
90% | UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence). |
50% | UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster. |