Extended Data Fig. 8: In vivo remodeling effects of cyclic D,L-α -peptides on the gut microbiota genera for which WD feeding caused significant changes in abundance compared to CHD.
From: Directed remodeling of the mouse gut microbiome inhibits the development of atherosclerosis
a, Comparison of the bacterial genera observed to significantly differ (adusted p-value < 0.1, as determined by DESeq2 using a two-sided Wald test with adjustment for multiple comparisons using the Benjamini-Hochberg method) between WD-fed mice compared to CHD-fed mice from two independent animal studies (in both studies, n = 9 animals for CHD group and n = 8 animals for WD group). 18 genera were observed to differ significantly in common between the two independent studies. Eleven of the 18 genera became significantly more abundant and 7 genera became significantly less abundant after WD-feeding for two weeks. b, Heatmap showing the fold change of each of the 18 genera identified in panel (a) that differed in both independent in vivo studies. c, Plot of the abundance changes for the 18 genera identified in panel (a) for WD-feeding relative to CHD-feeding (red indicates more abundant in WD-fed animals and blue indicates less abundant in WD-fed animals). d, A heat map showing how oral peptide treatment affected the abundance of the 18 genera identified in panel (a). The negative correlation for c[wLwReQeR] against the bacteria that became more abundant in WD-feeding indicates that c[wLwReQeR] remodeled the gut microbiome by causing those genera to become less abundant. In contrast, c[wLwKhShK] treatment promoted the growth of the bacterial genera that became less abundant with WD-feeding, as indicated the positive correlation for c[wLwKhShK] against WD-less abundant genera. All microbiota samples in this figure were taken from 2-wk feces samples of LDLr−/− mice.
