(Translated by https://www.hiragana.jp/)
Small Angle Scattering Biological Data Bank SASBDB Small Angle Scattering Biological Data Bank SASBDB

Curated repository for small angle scattering data and models


SASBDB currently contains:


4322

experimental data sets

5673

models
550 experimental data sets on hold
641 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDTZ8 – Streptococcus pyogenes Protein-ADP-ribose hydrolase: glycine cleavage system H-like protein complex

Glycine cleavage system H-like proteinProtein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) experimental SAS data
ALPHAFOLD model
Sample: Glycine cleavage system H-like protein monomer, 13 kDa Streptococcus pyogenes serotype … protein
Protein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) monomer, 30 kDa Streptococcus pyogenes serotype … protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% (v/v) glycerol, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 20
Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains Journal of Biological Chemistry :107770 (2024)
Ariza A, Liu Q, Cowieson N, Ahel I, Filippov D, Rack J
RgGuinier 2.4 nm
Dmax 8.4 nm
VolumePorod 53 nm3

Complex of BCL-6 corepressor ANK-linker-PUFD bound to Polycomb group RING finger protein 1 RAWUL, Lysine-specific demethylase 2B F-box-LRRs, and S-phase kinase-associated protein 1
Lysine-specific demethylase 2BPolycomb group RING finger protein 1S-phase kinase-associated protein 1BCL-6 corepressor experimental SAS data
Calcium modulates the tethering of BCOR-PRC1.1 enzymatic core to KDM2B via liquid-liquid phase separation. Commun Biol 2024 Sep 10;7(1):1112
Commun Biol 7(1):1112 (2024 Sep 10)
22mer poly-ADP ribose (PAR22) in 100mM NaCl
22mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 2024 Sep 10;15(1):7901
Nat Commun 15(1):7901 (2024 Sep 10)
Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, SEC-SAXS
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B 2024 Sep 09
The Journal of Physical Chemistry B (2024 Sep 09)
Small EDRK-rich factor 1 (SERF1a) bound to HTT-3 peptide
Isoform Short of Small EDRK-rich factor 1HTT3 experimental SAS data
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ 2024 Sep 1;
IUCrJ (2024 Sep 1)
Nearly full-length monomeric Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) (7-1110)
Ubiquitin carboxyl-terminal hydrolase 8 experimental SAS data
Autoinhibition of ubiquitin-specific protease 8: insights into domain interactions and mechanisms of regulation Journal of Biological Chemistry 2024 Aug :107727
Journal of Biological Chemistry :107727 (2024 Aug)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.