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The sex workers - who gathered in the Indian city of Calcutta - said they would set up a network to prevent women being targeted by trafficking gangs.
The network would also help to improve the social status of sex workers, according to conference organiser Smarajit Jena.
"It will also help fight for other social and economic causes of the sex workers like respectability in society and trade union rights," she said.
At risk
The network would be run through a special website, and would focus on women at risk in India, Nepal, Bangladesh, Cambodia, Thailand and Vietnam.
It is not clear exactly how many women are vulnerable.
However, Ms Jena said that evidence from HIV programmes across South Asia showed that several thousand women - particularly teenagers - were being targeted by trafficking gangs.
 Legalisation remains a key demand
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It included exhibitions and seminars on the plight of sex workers in various countries.
Legalisation
It was hosted by the Calcutta sex workers' union, called the Durbar Mahila Sammanoy Samity.
The union, with a membership of more than 5,000 active sex workers, has been at the forefront of a movement seeking to legalise the trade.
Many politicians belonging to different parties offered their support.
However, it also attracted criticism from some quarters in West Bengal, who said the sex trade helped spread AIDS.
Critics also argue that legalising the trade would lead to a rush of minors into the profession.
The BBC's Subir Bhaumik in Calcutta says that observers believe the idea of a network to combat trafficking may help allay fears about the numbers of minors in the sex trade.
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