File:ErbB2-Dependent-Chemotaxis-Requires-Microtubule-Capture-and-Stabilization-Coordinated-by-Distinct-pone.0055211.s008.ogv

English: Inhibition of PI3K leads to microtubule disassembly. SKBr3 cells expressing EGFP-tubulin αあるふぁ were grown on collagen-coated glass coverslips. Cells were then pre-treated with solvent (left) or the PI3K inhibitor LY294004 (right) for 1 h, before addition of 5 nM HRGβべーた1 and observation using the 63× objective (plan apochromat NA 1.4) of a fluorescence microscope (Zeiss Axiovert 200). Images were acquired every 4 min for 90 min using a Coolsnap HQ digital camera. Microtubules are stable over the time of the experiment in the control cells, while microtubules are disassembled starting ∼30 min after HRG addition when PI3K is inhibited. Corresponds to Fig. 6A.