Hippocampal LMNA Gene Expression is Increased in Late-Stage Alzheimer’s Disease
Abstract
:1. Introduction
2. Results
2.1. Lamin mRNA Levels across Alzheimer’s Disease Stages in the Human Hippocampus
2.2. Western-Blot of Lamin A/C in the Human Hippocampus Affected by AD
2.3. Correlation between Lamin Genes mRNA Levels and p-Tau Burden
2.4. Correlation between Lamin Genes mRNA Levels and Age
3. Discussion
4. Materials and Methods
4.1. Human Hippocampal Samples and Neuropathological Examination
4.2. Lamin mRNA Expression Analysis by RT-qPCR
4.3. Progerin mRNA Expression Analysis by RT-PCR
4.4. LMNA Protein Expression Analysis by Western Blot
4.5. Quantitative Assessment of P-Tau Deposits in Hippocampal Samples
4.6. Statistical Data Analysis
4.7. Ethics Approval and Consent to Participate
Supplementary Materials
Author Contributions
Funding
Acknowledgements
Conflicts of Interest
References
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Total Sample Set (n = 44) | Controls (n = 16) | AD Patients (n = 28) | ||||
---|---|---|---|---|---|---|
Gene | r Coefficient | p-Value | r Coefficient | p-Value | r Coefficient | p-Value |
LMNA | 0.254 | 0.092 | 0.581* | 0.018 | 0.002 | 0.990 |
LMNB1 | 0.211 | 0.164 | 0.149 | 0.582 | 0.358 | 0.061 |
LMNB2 | 0.222 | 0.143 | 0.396 | 0.129 | 0.165 | 0.401 |
ABC Score Staging | Controls (n = 16) | ABC Low (n = 14) | ABC Intermediate (n = 6) | ABC High (n = 8) |
---|---|---|---|---|
Age at death (years) | 56.1 ± 21.6 | 78.6 ± 9.4 | 88.3 ± 6.6 | 81.2 ± 12 |
Gender (Female) % | 43.8 | 64.3 | 66.7 | 62.5 |
PMI (h) | 7.5 ± 4.6 | 8.3 ± 8.6 | 4.9 ± 2.9 | 6.3 ± 4.4 |
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Méndez-López, I.; Blanco-Luquin, I.; Sánchez-Ruiz de Gordoa, J.; Urdánoz-Casado, A.; Roldán, M.; Acha, B.; Echavarri, C.; Zelaya, V.; Jericó, I.; Mendioroz, M. Hippocampal LMNA Gene Expression is Increased in Late-Stage Alzheimer’s Disease. Int. J. Mol. Sci. 2019, 20, 878. https://doi.org/10.3390/ijms20040878
Méndez-López I, Blanco-Luquin I, Sánchez-Ruiz de Gordoa J, Urdánoz-Casado A, Roldán M, Acha B, Echavarri C, Zelaya V, Jericó I, Mendioroz M. Hippocampal LMNA Gene Expression is Increased in Late-Stage Alzheimer’s Disease. International Journal of Molecular Sciences. 2019; 20(4):878. https://doi.org/10.3390/ijms20040878
Chicago/Turabian StyleMéndez-López, Iván, Idoia Blanco-Luquin, Javier Sánchez-Ruiz de Gordoa, Amaya Urdánoz-Casado, Miren Roldán, Blanca Acha, Carmen Echavarri, Victoria Zelaya, Ivonne Jericó, and Maite Mendioroz. 2019. "Hippocampal LMNA Gene Expression is Increased in Late-Stage Alzheimer’s Disease" International Journal of Molecular Sciences 20, no. 4: 878. https://doi.org/10.3390/ijms20040878