Flavonoid 3',5'-hydroxylase (EC 1.14.14.81 was wrongly classified as EC 1.14.13.88 in the past) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase.[1][2][3] This enzyme catalyses the following chemical reaction
| Flavonoid 3',5'-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.81 | ||||||||
| CAS no. | 94047-23-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- flavanone + 2 NADPH + 2 H+ + 2 O2 3',5'-dihydroxyflavanone + 2 NADP+ + 2 H2O (overall reaction)
- (1a) flavanone + NADPH + H+ + O2 3'-hydroxyflavanone + NADP+ + H2O
- (1b) 3'-hydroxyflavanone + NADPH + H+ + O2 3',5'-dihydroxyflavanone + NADP+ + H2O
Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).
References
edit- ^ Menting J, Scopes RK, Stevenson TW (October 1994). "Characterization of Flavonoid 3[prime],5[prime]-Hydroxylase in Microsomal Membrane Fraction of Petunia hybrida Flowers". Plant Physiology. 106 (2): 633–642. doi:10.1104/pp.106.2.633. PMC 159570. PMID 12232356.
- ^ Shimada Y, Nakano-Shimada R, Ohbayashi M, Okinaka Y, Kiyokawa S, Kikuchi Y (November 1999). "Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants(1)". FEBS Letters. 461 (3): 241–5. doi:10.1016/s0014-5793(99)01425-8. PMID 10567704.
- ^ de Vetten N, ter Horst J, van Schaik HP, de Boer A, Mol J, Koes R (January 1999). "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors". Proceedings of the National Academy of Sciences of the United States of America. 96 (2): 778–83. doi:10.1073/pnas.96.2.778. PMC 15213. PMID 9892710.
External links
edit- Flavanoid+3',5'-hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
