Ruppy
Ruppy (short for Ruby Puppy) is the world's first transgenic dog [1]. A cloned beagle, Ruppy and four other beagles produce a fluorescent protein that glows red upon excitation with ultraviolet light [2]. Ruppy was created in 2009 by a group of scientists in South Korea, led by Byeong-Chun Lee. The dog was cloned using viral transfection of fibroblasts cells with a protein that expresses the red fluorescent gene. The nucleus of the transfected fibroblast was then inserted into the enucleated oocyte of another dog cell, leading to generation of dog oocytes expressing the red fluorescent protein. These cloned embryos were then implanted into the uterus of a surrogate mother [1].