EF手しゅ

EF手しゅ
重じゅう组的第だい四よん双そう小しょう核かく草履虫ぞうりむし钙调蛋白たんぱく结构
鑑定かんてい
標しるべ誌し	efhand
Pfam	PF00036（旧版きゅうばん）
InterPro	IPR002048
PROSITE	PDOC00018
SCOP	1osa / SUPFAM
OPM蛋白たんぱく	1djx
現有げんゆう可用かよう的てき蛋白たんぱく結構けっこう： Pfam 結構けっこう（旧版きゅうばん） / ECOD PDB RCSB PDB; PDBe; PDBj PDBsum 結構けっこう概要がいよう PDB 1s6jA:375-402 1s6iA:375-403 1m39A:141-169 2a4jA:141-169 1oqpA:138-166 2bbnA:121-148 2bkhB:121-148 1mxeA:121-148 4cln :121-148 2bbmA:121-148 1cdmA:121-146 1lin :121-148 2bkiB:121-148 1oojA:121-148 1osa :121-148 1clm :121-147 1n0yB:121-148 1exrA:121-148 1rfjA:121-149 1ggzA:121-148 1ak8 :48-75 1sw8A:48-76 1j7oA:48-76 1f70A:48-76 1r2uA:56-84 1r6pA:56-84 1j1eA:56-84

EF手しゅ（英語えいご：EF hand）是ぜ一いち种在钙结合あい蛋白たんぱく中ちゅう发现的てき螺旋らせん-环-螺旋らせん结构域いき或ある结构基もと序じょ。

EF手て包括ほうかつ一いち个螺旋らせん-环-螺旋らせん拓つぶせ扑结构，就像人じん类的手しゅ上じょう拇指ぼし和食わしょく指ゆび，而Ca²⁺离子与あずか其配はい体たい就位しゅうい于环内ない。这一结构最早もはや在ざい小清こせい蛋白たんぱく中ちゅう发现，小清こせい蛋白たんぱく含有がんゆう三さん个EF手て结构，与あずか钙离子こ结合而造成ぞうせい的てき肌はだ肉にく放ひ松まつ有ゆう关。

EF手て包含ほうがん两个αあるふぁ螺旋らせん，由よし一いち个短的てき（通常つうじょう包括ほうかつ12个氨基酸さん）环状区域くいき连接，常用じょうよう于结合あい钙离子。EF手しゅ也出现在钙调蛋白たんぱく的てき所有しょゆう结构域いき和かず肌はだ肉にく蛋白たんぱく质肌はだ钙蛋白しろC中なか。

分ぶん类

• 自じ从EF手て结构模も体たい在ざい1973年ねん被ひ描绘以来いらい，迄まで今こん为止该家族かぞく的てき蛋白たんぱく质已扩展到いた至いたり少しょう66个亚家族かぞく。EF手しゅ结构基もと元もと可か以分为Canonical EF-hands和わPseudo EF-hands 两大类：

EF手て蛋白たんぱく家族かぞく的てき系けい统发育そだて树

亚族

• EPS15 homology (EH) domain – IPR000261

例れい子こ

人ひと类基因いん组中なか，包つつみ含有がんゆうEF手て的てき蛋白たんぱく质有：

• ACTN1、ACTN2、ACTN3、ACTN4、APBA2BP; AYTL1; AYTL2
• C14orf143; CABP1; CABP2; CABP3; CABP4; CABP5; CABP7; CALB1; CALB2; CALM2; CALM3; CALML3; CALML4; CALML5; CALML6; CALN1; CALU; CAPN1; CAPN11; CAPN2; CAPN3; CAPN9; CAPNS1; CAPNS2; CAPS; CAPS2; CAPSL; CBARA1; CETN1; CETN2; CETN3; CHP; CHP2; CIB1; CIB2; CIB3; CIB4; CRNN
• DGKA; DGKB; DGKG; DST; DUOX1; DUOX2
• EFCAB1; EFCAB2; EFCAB4A; EFCAB4B; EFCAB6; EFCBP1; EFCBP2; EFHA1; EFHA2; EFHB; EFHC1; EFHD1; EFHD2; EPS15; EPS15L1
• FKBP10; FKBP14; FKBP7; FKBP9; FKBP9L; FREQ; FSTL1; FSTL5
• GCA; GPD2; GUCA1A; GUCA1B; GUCA1C
• hippocalcin; HPCAL1; HPCAL4; HZGJ
• IFPS; ITSN1; ITSN2; KCNIP1; KCNIP2; KCNIP3; KCNIP4; KIAA1799
• LCP1
• MACF1; MRLC2; MRLC3; MST133; MYL1; MYL2; MYL5; MYL6B; MYL7; MYL9; MYLC2PL; MYLPF
• NCALD; NIN; NKD1; NKD2; NLP; NOX5; NUCB1; NUCB2
• OCM
• PDCD6; PEF1; PKD2; PLCD1; PLCD4; PLCH1; PLCH2; PLS1; PLS3; PP1187; PPEF1; PPEF2; PPP3R1; PPP3R2; PRKCSH; PVALB
• RAB11FIP3; RASEF; RASGRP; RASGRP1; RASGRP2; RASGRP3; RCN1; RCN2; RCN3; RCV1; RCVRN; REPS1; RHBDL3; RHOT1; RHOT2; RPTN; RYR2; RYR3
• S100A1; S100A11; S100A12; S100A6; S100A8; S100A9; S100B; S100G; S100Z; SCAMC-2; SCGN; SCN5A; SDF4; SLC25A12; SLC25A13; SLC25A23; SLC25A24; SLC25A25; SPATA21; SPTA1; SPTAN1; SRI
• TBC1D9; TBC1D9B; TCHH; TESC; TNNC1; TNNC2
• USP32
• VSNL1
• ZZEF1

另见

• 锚定结构域いき，另一种由αあるふぁ-螺旋らせん组成的てき钙结合あい结构基もと序じょ。

延伸えんしん阅读

• Branden C, Tooze J. Chapter 2: Motifs of protein structure. Introduction to Protein Structure. New York: Garland Pub. 1999: 24–25. ISBN 0-8153-2305-0. 
• Nakayama S, Kretsinger RH. Evolution of the EF-hand family of proteins. Annu Rev Biophys Biomol Struct. 1994, 23: 473–507. PMID 7919790. doi:10.1146/annurev.bb.23.060194.002353. 
• Zhou Y, Yang W, Kirberger M, Lee HW, Ayalasomayajula G, Yang JJ. Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins. Proteins. November 2006, 65 (3): 643–55. PMID 16981205. doi:10.1002/prot.21139. 
• Zhou Y, Frey TK, Yang JJ. Viral calciomics: interplays between Ca2+ and virus. Cell Calcium. July 2009, 46 (1): 1–17. PMID 19535138. doi:10.1016/j.ceca.2009.05.005. 
• Nakayama S, Moncrief ND, Kretsinger RH. Evolution of EF-hand calcium-modulated proteins. II. Domains of several subfamilies have diverse evolutionary histories. J. Mol. Evol. May 1992, 34 (5): 416–48. PMID 1602495. doi:10.1007/BF00162998. 
• Hogue CW, MacManus JP, Banville D, Szabo AG. Comparison of terbium (III) luminescence enhancement in mutants of EF hand calcium binding proteins. J. Biol. Chem. July 1992, 267 (19): 13340–7. PMID 1618836. 
• Bairoch A, Cox JA. EF-hand motifs in inositol phospholipid-specific phospholipase C. FEBS Lett. September 1990, 269 (2): 454–6. PMID 2401372. doi:10.1016/0014-5793(90)81214-9. 
• Finn BE, Forsén S. The evolving model of calmodulin structure, function and activation. Structure. January 1995, 3 (1): 7–11. PMID 7743133. doi:10.1016/S0969-2126(01)00130-7. 
• Stathopulos PB, Zheng L, Li GY, Plevin MJ, Ikura M. Structural and mechanistic insights into STIM1-mediated initiation of store-operated calcium entry. Cell. October 2008, 135 (1): 110–22. PMID 18854159. doi:10.1016/j.cell.2008.08.006. 
• Nelson MR, Thulin E, Fagan PA, Forsén S, Chazin WJ. The EF-hand domain: a globally cooperative structural unit. Protein Sci. February 2002, 11 (2): 198–205. PMC 2373453 . PMID 11790829. doi:10.1110/ps.33302. 

外部がいぶ链接

• Nelson M, Chazin W. EF-Hand Calcium-Binding Proteins Data Library. Vanderbilt University. [2009-08-29]. （原始げんし内容ないよう存そん档于2009-08-25）. 
• Haiech J. EF-hand protein database (EF-handome). European Calcium Society and the Université Libre de Bruxelles. [2009-08-29]. （原始げんし内容ないよう存そん档于2009-08-09）. upon request to haiech@pharma.u-strasbg.fr 
• Yang J. Calciomics. Georgia State University. [2009-08-29]. （原始げんし内容ないよう存そん档于2009-10-12）. prediction server for EF-hand calcium binding proteins